Mp3g20340



Description

Annotation

Database ID Description
ProSiteProfiles PS50222 EF-hand calcium-binding domain profile.
Pfam PF08022 FAD-binding domain
MobiDBLite mobidb-lite consensus disorder prediction
Gene3D G3DSA:3.40.50.80 -
SUPERFAMILY SSF52343 Ferredoxin reductase-like, C-terminal NADP-linked domain
CDD cd00051 EFh
Coils Coil Coil
SFLD SFLDS00052 Ferric Reductase Domain
ProSitePatterns PS00018 EF-hand calcium-binding domain.
Gene3D G3DSA:2.40.30.10 Translation factors
SUPERFAMILY SSF47473 EF-hand
FunFam G3DSA:1.10.238.10:FF:000049 Respiratory burst oxidase homolog A
Pfam PF08030 Ferric reductase NAD binding domain
SUPERFAMILY SSF63380 Riboflavin synthase domain-like
PANTHER PTHR11972 NADPH OXIDASE
Gene3D G3DSA:1.10.238.10 -
FunFam G3DSA:2.40.30.10:FF:000019 Respiratory burst oxidase homolog A
ProSiteProfiles PS51384 Ferredoxin reductase-type FAD binding domain profile.
Pfam PF01794 Ferric reductase like transmembrane component
PRINTS PR00466 Cytochrome B-245 heavy chain signature
Pfam PF08414 Respiratory burst NADPH oxidase
SFLD SFLDG01169 NADPH oxidase subgroup (NOX)
CDD cd06186 NOX_Duox_like_FAD_NADP
KEGG K13447 respiratory burst oxidase [EC:1.6.3.- 1.11.1.-]
KOG KOG0039 Ferric reductase, NADH/NADPH oxidase and related proteins; [PQ]
KOG KOG0034 Ca2+/calmodulin-dependent protein phosphatase (calcineurin subunit B), EF-Hand superfamily protein; [T]
MapolyID Mapoly0258s0001 -
GO GO:0005509 calcium ion binding
GO GO:0050664 oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor
GO GO:0005886 plasma membrane
GO GO:0016491 oxidoreductase activity
GO GO:0004601 peroxidase activity
GO GO:0016174 NAD(P)H oxidase H2O2-forming activity
GO GO:0016020 membrane

Nomenclature

Gene symbol Product Transcript ID Status
MpNOX1 NADPH oxidase Published
MpRBOH2 respiratory burst oxidase homolog (Rboh), nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) Published
MpRBOHB NADPH oxidase/respiratory burst oxidase homolog Published
MpNOX1 was assigned to Mp7g00270 until June 2026, but has now been corrected to Mp3g20340.
Mp3g20340 encodes an NADPH oxidase (NOX)/respiratory burst oxidase homolog (RBOH), which has been assigned different gene names in three papers: MpRBOHB in Hashimoto et al. (10.1111/ppl.14101, 2023), MpRBOH1 in Chu et al. (10.1016/j.cub.2023.01.050, 2023), and MpRBOH2 in Yotsui et al. 2023 (10.1016/j.cub.2023.07.068). It has also been referred to as MpNOX1 (Rico-Resendiz 2020 et al. 10.3390/ijms21218354; Spinedi et al. 2021. 10.3390/plants10071478).

Associated Literature (AI-based literature mining, Beta-version)

The single Marchantia NADPH oxidase (RBOH) required for chitin-induced apoplastic ROS production; directly phosphorylated by MpPBLa at conserved cytosolic N-terminal sites, which is essential for ROS production, demonstrating conservation of the PBL-RBOH immune module across land plants. NAMING NOTE: Mp3g20340 encodes a NADPH oxidase (NOX) / respiratory burst oxidase homolog (RBOH) that has been assigned different gene names in three papers: MpRBOHB in Hashimoto et al. (10.1111/ppl.14101, 2023), MpRBOH1 in Chu et al. (this paper, 10.1016/j.cub.2023.01.050, 2023), and MpRBOH2 in Yotsui et al. 2023 (10.1016/j.cub.2023.07.068); it has also been referred to as MpNOX2. Linked here by gene ID (Mp3g20340), not by name, because the name is ambiguous across papers.

4 core 5 peripheral

Yamashita Y, Hagiwara Y, Hashimoto K, Ogawa M, Hoshino S, Suzuki H, Iwano M, Ishikawa T, Nishihama R, Kuchitsu K. (2026) · Current biology : CB  research experimental subject
The second M. polymorpha RBOH; complete null and double-mutant analyses show MpRBOHA/MpRBOHB-derived ROS are indispensable for development and cuticle/cell-wall morphogenesis.
Hashimoto, T., et al. (2023) · Physiologia Plantarum  research experimental subject
Respiratory burst oxidase homolog B (NADPH oxidase; synonym MpRBOH1/MpRBOH2). Phosphorylation of conserved N-terminal serines enhances Ca2+ binding to its EF-hands, activating MpRBOHB for chitin-triggered ROS production.
Chu J, Monte I, DeFalco TA, Köster P, Derbyshire P, Menke FLH, Zipfel C. (2023) · Current Biology  research experimental subject
The single Marchantia NADPH oxidase (RBOH) required for chitin-induced apoplastic ROS production; directly phosphorylated by MpPBLa at conserved cytosolic N-terminal sites, which is essential for ROS production, demonstrating conservation of the PBL-RBOH immune module across land plants. NAMING NOTE: Mp3g20340 encodes a NADPH oxidase (NOX) / respiratory burst oxidase homolog (RBOH) that has been assigned different gene names in three papers: MpRBOHB in Hashimoto et al. (10.1111/ppl.14101, 2023), MpRBOH1 in Chu et al. (this paper, 10.1016/j.cub.2023.01.050, 2023), and MpRBOH2 in Yotsui et al. 2023 (10.1016/j.cub.2023.07.068); it has also been referred to as MpNOX2. Linked here by gene ID (Mp3g20340), not by name, because the name is ambiguous across papers.
Rico-Reséndiz, F., et al. (2020) · International Journal of Molecular Sciences  research experimental subject
NADPH oxidase producing reactive oxygen species. Up-regulated at 12 and 150 HPT and down-regulated at 24 HPT, suggesting Pi limitation promotes superoxide production.
Tabassum N, Goodrich J, Loake GJ. (2026) · Journal of experimental botany  research citation background
Respiratory Burst Oxidase Homolog 1, a key ROS-production component, referenced for evolutionary conservation of the defence module.

Transcript models

Transcript ID Location Sequences Extract region
Mp3g20340.1 chr3:22237262..22244107 (+) Gene /  mRNA /  CDS /  Protein
FASTA / GenBank (with flanking bases)

Expression Level powered by MBEX

Link to the original image in MBEX

Single-cell expression scRNA-seq atlas from Wang et al., 2023

Expression of Mp3g20340 across the single-cell RNA-seq atlas. Cluster identities (right) annotate the same UMAP used for the feature plot (left).

Feature plot of Mp3g20340 on UMAP
Feature plot (Seurat scale expression level).
UMAP cluster annotation
Cell-type annotation of the UMAP clusters.
Violin plot by developmental stage
Violin plot by stage. Expression across developmental time points (D0–D31).
Violin plot by cell cluster
Violin plot by cluster. Expression across cell clusters (1–21).

Gene structure:

Sequences:

Gene UTR + CDS + intron

FASTA

mRNA UTR + CDS

FASTA

CDS

FASTA

Protein

FASTA


MarpolBase / Genome Informatics Lab. NIG.