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CasFinder: Guide RNA design for the CRISPR/Cas9 system
Genome
Mp JGI 3.1
MpTak1 v5.1
Search conditions:
Length of gRNA: 18 nt, Seed Region: 9 nt, Primary/secondary PAM: NGG/NAG
Length of gRNA: 20 nt, Seed Region: 8 nt, Primary/secondary PAM: NGG/NAG
Enter your sequence(s) in FASTA format below. (Please make your sequence as short as possible!
Maximum=1,000bp
)
Options:
Filter out T-strings with this or greater length:
Input sequence is exogeneous.
Force search of repeat regions in input sequences
Reference:
Aach J, Mali P, Church GM. 2014. CasFinder: Flexible algorithm for identifying specific Cas9 targets in genomes. bioRxiv doi: 10.1101/005074