Toggle navigation
Genome browser
JBrowse
WebApollo (login required)
Tools
BLAST (MpTak1v5.1)
BLAST (JGI 3.1 and plant species)
GMAP - cDNA alignment
CasFinder
Utilities
Genome Slicer
Gene Fetcher
Gene ID converter
External tools
GGGenome
CRISPRdirect
Nomenclature
Archives
Download
Nuclear genome (MpTak1v5.1)
Nuclear genome (JGI 3.1 )
M. polymorpha subsp. polymorpha BR5 (MppBR5)
M. polymorpha subsp. montivagans SA2 (MpmSA2)
Organellar genomes (Kitashirakawa-2)
About
About us
Genomes
FAQ
CasFinder: Guide RNA design for the CRISPR/Cas9 system
Genome
Mp JGI 3.1
MpTak1 v5.1
Search conditions:
Length of gRNA: 18 nt, Seed Region: 9 nt, Primary/secondary PAM: NGG/NAG
Length of gRNA: 20 nt, Seed Region: 8 nt, Primary/secondary PAM: NGG/NAG
Enter your sequence(s) in FASTA format below. (Please make your sequence as short as possible!
Maximum=1,000bp
)
Options:
Filter out T-strings with this or greater length:
Input sequence is exogeneous.
Force search of repeat regions in input sequences
Reference:
Aach J, Mali P, Church GM. 2014. CasFinder: Flexible algorithm for identifying specific Cas9 targets in genomes. bioRxiv doi: 10.1101/005074