Mp7g08500



Description

Annotation

Database ID Description
MobiDBLite mobidb-lite consensus disorder prediction
Coils Coil Coil
SUPERFAMILY SSF55073 Nucleotide cyclase
CDD cd07302 CHD
SUPERFAMILY SSF109604 HD-domain/PDEase-like
CDD cd00077 HDc
ProSitePatterns PS00126 3'5'-cyclic nucleotide phosphodiesterase domain signature.
Gene3D G3DSA:3.30.70.1230 Nucleotide cyclase
Pfam PF00211 Adenylate and Guanylate cyclase catalytic domain
PRINTS PR00387 3'5'-cyclic nucleotide phosphodiesterase signature
Gene3D G3DSA:1.10.1300.10 -
SMART SM00044 cyc_6
Pfam PF00233 3'5'-cyclic nucleotide phosphodiesterase
ProSiteProfiles PS50125 Guanylate cyclase domain profile.
ProSiteProfiles PS51845 3'5'-cyclic nucleotide phosphodiesterase domain profile.
SMART SM00471 hd_13
PANTHER PTHR43336 OXYGEN SENSOR HISTIDINE KINASE RESPONSE REGULATOR DEVS/DOSS
KOG KOG3689 Cyclic nucleotide phosphodiesterase; N-term missing; [T]
KOG KOG2133 Transcriptional corepressor Atrophin-1/DRPLA; C-term missing; [R]
MapolyID Mapoly0068s0004 -
GO GO:0009190 cyclic nucleotide biosynthetic process
GO GO:0008081 phosphoric diester hydrolase activity
GO GO:0035556 intracellular signal transduction
GO GO:0004114 3',5'-cyclic-nucleotide phosphodiesterase activity
GO GO:0007165 signal transduction

Nomenclature

Gene symbol Product Transcript ID Status
MpCAPE adenylyl cyclase with a phosphodiestrase domain Published

Associated Literature (AI-based literature mining, Beta-version)

Cloned from Marchantia as a fusion protein with an N-terminal cyclic-nucleotide phosphodiesterase domain and a C-terminal class III adenylyl cyclase domain; recombinant GST-MpCAPE-AC produced cAMP (Mn2+ enhancing activity ~35-fold over Mg2+) and complemented an E. coli AC-deficient mutant, while His-MpCAPE-PDE hydrolyzed cAMP/cGMP, with catalytic residues (Asp-1340 for AC; His-199/203 for PDE) confirmed essential by mutagenesis. GUS reporter showed expression in the antheridium, implicating it in male reproductive development.

4 core 1 peripheral

Yamamoto, C., et al. (2024) · Proceeding of the National Academy of Sciences USA  research experimental subject
Dual adenylyl cyclase/phosphodiesterase that synthesizes and hydrolyzes cAMP, expressed during maturation of male reproductive organs. Knockout mutants are male-infertile with impaired flagellar motility, swimming in circles instead of straight (16.4 vs 43.5 um/s WT). Complementation restored normal motility, identifying it as the central cAMP regulator of sperm motility.
Hayashida, Y., et al. (2021) · Journal of Plant Research  research experimental subject
Bifunctional adenylyl cyclase / cAMP phosphodiesterase (CAPE); its PDE domain (MpCAPE-PDE) was purified from E. coli and kinetically characterized (Km/Vmax), establishing cAMP-hydrolyzing activity alongside its AC activity.
Yamamoto, C., et al. (2021) · Scientific Reports  research experimental subject
Marchantia CAPE encoding combined class III adenylyl cyclase (C-terminal) and class I phosphodiesterase (N-terminal) domains; biochemical assays confirmed both domains are enzymatically active, with cAMP-specific PDE and a low-activity high-affinity AC. Shown to be specifically expressed in spermatogenous cells of antheridia but not vegetative organs, serving as the functional reference for CAPE in motile-sperm streptophytes.
Kasahara, M., et al. (2016) · Scientific Reports  research experimental subject
Cloned from Marchantia as a fusion protein with an N-terminal cyclic-nucleotide phosphodiesterase domain and a C-terminal class III adenylyl cyclase domain; recombinant GST-MpCAPE-AC produced cAMP (Mn2+ enhancing activity ~35-fold over Mg2+) and complemented an E. coli AC-deficient mutant, while His-MpCAPE-PDE hydrolyzed cAMP/cGMP, with catalytic residues (Asp-1340 for AC; His-199/203 for PDE) confirmed essential by mutagenesis. GUS reporter showed expression in the antheridium, implicating it in male reproductive development.
Ren, X., et al. (2024) · Plant Cell  research experimental tool
Antheridium-expressed gene used as a marker; activated in MpGLID-overexpressing transgenics.

Transcript models

Transcript ID Location Sequences Extract region
Mp7g08500.1 chr7:11489973..11502337 (-) Gene /  mRNA /  CDS /  Protein
FASTA / GenBank (with flanking bases)

Expression Level powered by MBEX

Link to the original image in MBEX

Single-cell expression scRNA-seq atlas from Wang et al., 2023

Expression of Mp7g08500 across the single-cell RNA-seq atlas. Cluster identities (right) annotate the same UMAP used for the feature plot (left).

Feature plot of Mp7g08500 on UMAP
Feature plot (Seurat scale expression level).
UMAP cluster annotation
Cell-type annotation of the UMAP clusters.
Violin plot by developmental stage
Violin plot by stage. Expression across developmental time points (D0–D31).
Violin plot by cell cluster
Violin plot by cluster. Expression across cell clusters (1–21).

Gene structure:

Sequences:

Gene UTR + CDS + intron

FASTA

mRNA UTR + CDS

FASTA

CDS

FASTA

Protein

FASTA


MarpolBase / Genome Informatics Lab. NIG.