Mp8g11800



Description

Annotation

Database ID Description
Coils Coil Coil
SMART SM00504 Ubox_2
SUPERFAMILY SSF57850 RING/U-box
CDD cd21037 MLKL_NTD
SUPERFAMILY SSF48371 ARM repeat
Gene3D G3DSA:3.30.40.10 Zinc/RING finger domain, C3HC4 (zinc finger)
SMART SM00185 arm_5
Pfam PF04564 U-box domain
Pfam PF19584 MCAfunc domain
ProSiteProfiles PS51698 U-box domain profile.
Gene3D G3DSA:1.25.10.10 -
CDD cd16664 RING-Ubox_PUB
PANTHER PTHR45958 RING-TYPE E3 UBIQUITIN TRANSFERASE
KEGG K08332 vacuolar protein 8
KOG KOG4224 Armadillo repeat protein VAC8 required for vacuole fusion, inheritance and cytosol-to-vacuole protein targeting; C-term missing; [U]
KOG KOG0166 Karyopherin (importin) alpha; N-term missing; C-term missing; [U]
MapolyID Mapoly0008s0036 -
GO GO:0004842 ubiquitin-protein transferase activity
GO GO:0005515 protein binding
GO GO:0016567 protein ubiquitination

Nomenclature

Gene symbol Product Transcript ID Status
MpNOP1 Plant U-box E3 Ubiquitin Ligase NOP1 Published

Associated Literature (AI-based literature mining, Beta-version)

Plant U-box E3 Ubiquitin Ligase NOP1; NOP1 has an essential role in schizogenous intercellular space formation in Marchantia polymorpha.NOP1 encodes a Plant U-box (PUB) E3 ubiquitin ligase carrying tandem ARMADILLO (ARM) repeats in the C terminus. An in vitro ubiquitination assay indicated that the NOP1 protein possesses E3 ubiquitin ligase activity in a U-box-dependent manner. Confocal microscopy and biochemical analysis showed that NOP1 was localized to the plasma membrane. (from nomenclature)

2 core 4 peripheral

Sugano, S. S., et al. (2018) · PLoS ONE  research experimental subject
Targeted to assess editing efficiency via the easily scored transparent-thallus (air-chamber-less) phenotype; 87.5% of 32 T1 plants were mutated. Used to test guide length (17nt or fewer dramatically less efficient) and to induce 0.5/1.5/4.5kb deletions with paired gRNAs; no off-target mutations.
Kopischke, S., et al. (2017) · Plant Methods  research experimental subject
Selected as the TALEN knockout target because its mutant lacks air chamber formation (an easily traceable phenotype); both TALEN approaches achieved >20% targeting efficiency with deletions of 4 nt to ~2000 nt across 22 sequenced T1 lines. Two mutants carried in-frame indels revealing novel Mpnop1-related phenotypes, with stable inheritance across generations.
Lim, C. J., et al. (2024) · New Phytologist  research citation background
Alternative name for MpPUB24/NOPPERABO1, previously reported to control formation of intercellular spaces.
Ishizaki, K. (2015) · Frontiers in Plant Science  review citation background
NOPPERABO1; PUB E3 ligase required for intercellular space formation
Althoff, F., et al. (2022) · Fronteirs in Plant Science  research citation comparator
Plant U-box gene controlling air pore formation

Transcript models

Transcript ID Location Sequences Extract region
Mp8g11800.1 chr8:15294953..15302422 (-) Gene /  mRNA /  CDS /  Protein
FASTA / GenBank (with flanking bases)
Mp8g11800.2 chr8:15294953..15302422 (-) Gene /  mRNA /  CDS /  Protein
FASTA / GenBank (with flanking bases)

Expression Level powered by MBEX

Link to the original image in MBEX

Single-cell expression scRNA-seq atlas from Wang et al., 2023

Expression of Mp8g11800 across the single-cell RNA-seq atlas. Cluster identities (right) annotate the same UMAP used for the feature plot (left).

Feature plot of Mp8g11800 on UMAP
Feature plot (Seurat scale expression level).
UMAP cluster annotation
Cell-type annotation of the UMAP clusters.
Violin plot by developmental stage
Violin plot by stage. Expression across developmental time points (D0–D31).
Violin plot by cell cluster
Violin plot by cluster. Expression across cell clusters (1–21).

Gene structure:

Sequences:

Gene UTR + CDS + intron

FASTA

mRNA UTR + CDS

FASTA

CDS

FASTA

Protein

FASTA

Gene structure:

Sequences:

Gene UTR + CDS + intron

FASTA

mRNA UTR + CDS

FASTA

CDS

FASTA

Protein

FASTA

MarpolBase / Genome Informatics Lab. NIG.