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Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies.

Janssen B, Romein R, van den Berg WAM, Lindhoud S, Borst JW, van Mierlo CPM. · 2026 · FEBS Open Bio   research

doi:10.1002/2211-5463.70313   PMID:42482459   PMC13398774

Linked genes (3)  1 core 2 peripheral

Gene ID Name Evidence / Role Function (this paper)
Mp4g11820 MpARF2 experimental subject Auxin response factor that forms nuclear assemblies in vivo, possibly by liquid-liquid phase separation. The paper reports a three-step procedure yielding soluble, monomeric full-length MpARF2 and truncated variants, plus a preparation step giving reproducible in vitro phase separation.
Mp1g12750 MpARF1 experimental comparator Referenced as another Marchantia ARF alongside MpARF2 in the purification work.
Mp1g07070 MpARF3 experimental comparator Referenced as another Marchantia ARF alongside MpARF2 in the purification work.