MarpolBase - Rapid plastid isolation reveals the chloroplast proteome and structures of the chlororibosome large subunit and RuBisCO in Marchantia polymorpha.

Rapid plastid isolation reveals the chloroplast proteome and structures of the chlororibosome large subunit and RuBisCO in Marchantia polymorpha.

Raval PK, Mitchell C, Lozano-Quiles M, O'Keefe S, Nyman TA, Battersby B, Butcher SJ, Gould SB. · 2026 · bioRxiv research

Gene ID	Name	Evidence / Role	Function (this paper)
Mp2g15420	Mp2g15420 missing	experimental tool	Light-harvesting complex A1 (LHCA1), used as a citrine-tagged plastid marker to validate the rapid plastid isolation protocol. Colocalisation of the reporter with chlorophyll autofluorescence confirmed the purified fraction was intact plastids.
Mp3g01370	Mp3g01370 missing	sequence background	Plastid protein of unknown function identified in the M. polymorpha plastid proteome. AlphaFold3 modelling followed by Dali search assigned it a PsbQ-like structure, one of only two of thirteen uncharacterised plastid proteins given a structural match.
Mp5g18410	Mp5g18410 missing	sequence background	Plastid protein of unknown function identified in the M. polymorpha plastid proteome. Structure prediction and Dali search matched it to an AFMP4P virulence factor fold; eleven further plastid proteins remain without any structural assignment.