Mp7g07270
Description
Annotation
| Database | ID | Description |
|---|---|---|
| Pfam | PF05498 | Rapid ALkalinization Factor (RALF) |
| PANTHER | PTHR33136 | RAPID ALKALINIZATION FACTOR-LIKE |
| MapolyID | Mapoly0076s0067 | - |
Nomenclature
| Gene symbol | Product | Transcript ID | Status |
|---|---|---|---|
| MpRALF1 | cysteine-rich peptide RALF1 | Published |
Associated Literature (AI-based literature mining, Beta-version)
Sperm-derived RAPID ALKALINIZATION FACTOR peptide, the central subject of this study. Identified in a screen of the top 1,000 antheridia-expressed genes, MpRALF1 is specifically and highly expressed in mature antheridia (RT-qPCR, proMpRALF1:GUS) and the peptide accumulates in spermatozoid flagella. Three CRISPR/Cas9 frameshift knockout lines and amiRNA knockdown (Ri-MpRALF1) reduced male fertility (fewer sporophytes after crossing), lowered sperm ROS levels and impaired sperm kinematics (reduced curvilinear velocity, fewer rapidly progressive and more slow/non-progressive spermatozoids); 10 nM synthetic MpRALF1 peptide rescued both ROS and motility. MpRALF1 is perceived by the MpFER-MpLLG receptor complex (pulldown, CoIP, luciferase complementation), and its N-terminal 18-aa region binds MpFER whereas its C-terminal 38-aa region binds MpLLG. NAMING NOTE: gene ID stated in the paper; Mecchia et al. 2022 (Development, doi:10.1242/dev.200580) calls this same locus MpRALF3 and uses MpRALF1 for Mp1g27120.
1 core 1 peripheral
Zhang, Y., et al. (2026)
· Nature Plants research experimental subject
Sperm-derived RAPID ALKALINIZATION FACTOR peptide, the central subject of this study. Identified in a screen of the top 1,000 antheridia-expressed genes, MpRALF1 is specifically and highly expressed in mature antheridia (RT-qPCR, proMpRALF1:GUS) and the peptide accumulates in spermatozoid flagella. Three CRISPR/Cas9 frameshift knockout lines and amiRNA knockdown (Ri-MpRALF1) reduced male fertility (fewer sporophytes after crossing), lowered sperm ROS levels and impaired sperm kinematics (reduced curvilinear velocity, fewer rapidly progressive and more slow/non-progressive spermatozoids); 10 nM synthetic MpRALF1 peptide rescued both ROS and motility. MpRALF1 is perceived by the MpFER-MpLLG receptor complex (pulldown, CoIP, luciferase complementation), and its N-terminal 18-aa region binds MpFER whereas its C-terminal 38-aa region binds MpLLG. NAMING NOTE: gene ID stated in the paper; Mecchia et al. 2022 (Development, doi:10.1242/dev.200580) calls this same locus MpRALF3 and uses MpRALF1 for Mp1g27120.
Mecchia, M.A., et al. (2022)
· Development research sequence comparator
One of the three M. polymorpha RALF peptide genes analysed as candidate MpFER ligands; clusters with the AtRALF1 subgroup and carries the RRXL motif for S1P processing. NAMING NOTE: the MpRALF1/MpRALF3 numbering used here is swapped relative to the MarpolBase nomenclature and to Zhang et al. 2026 (Nat. Plants), in which this locus (Mp7g07270) is MpRALF1. Linked by the ID stated in this paper.
Single-cell expression scRNA-seq atlas from Wang et al., 2023
Expression of Mp7g07270 across the single-cell RNA-seq atlas. Cluster identities (right) annotate the same UMAP used for the feature plot (left).
Gene structure:
Sequences: |
Gene UTR + CDS + intron |
| >Mp7g07270.1 AAGTGAGAAG CCGTGTTCAG TAGCGAGCAA GGTGAGAGCA GAGAGCGAGT CGTTAGCACG TCGATACATT GTCTTCTTGC AAGGCGGTCA TTCGTCTTCT TCGTCGTTCT TCAGGGCTTG CGGAGGGCAG TTTTCTTGAA GGCGTCCTCC AGCTCGAATT TAGTGTTCGT GTGCGTGGTG TTTGATCGGC GAAGGGAGAG ATTGACTGTG TGTTTGTGGG AGAGAGAGAG AGAGAGAGAG AGAGAGAGGA GCTCTTTGTG AGCCGGCCTG GCAGGCAAGG GCGAGGAAGG TAATCGAGGC CTTGTTCGCG GGCACCGGCG TGGCTTTAGT CGGTCTCGAG GGAGGAGCAG CAGCAGGAGC AGTTGGAGCG ATGGGGCGTT GTGGAATGCA GAGAACAAAT GTTTGGTTTT TGGTCCTTCT ATCGGTGTTA CTACTGATGG GGGTAGCGCA AGCCTCTTTT GACATGAGTT CTGAGACCTT CGTCCCACAG AGGACTGCTT CGTTGTCGAA TCAACTGGAC TCAGAGGTGA CTAGAAGAAT GCTGGCCGCC TCAGGGTACT ACGTCGGATA CGGTGCCTTA ACTGCCAATC GTGTTCCTTG CCCGCCTCAG TCGGGACGAT CGTATTACAC TCCCGGGTGC TCGACTGCTT CGGGACCAGT TCGGCCTTAC ACCAGAGGAT GCTCGACTAT CACCCGGTGT GCTAGGGATG GCTAGGTGCT TCGTTGAGAT TTCAAGGATG TCCGTTGACC TCCCTATTAT TTTCGGGGGA AGCCTGTTTG TTCCTTCCCT CCCACTGGAA AGAGGAACTA TTGTGAAGTG TTCGGGTTTC TCTCTCTTCT GCGCGGGGTG TTGGTGCTAA TACTGAAAGT CGGCAAACTC TCAAATAAAC CTTAAATAGT AAGAAAAAAA AGAAGTATAT TTCAGTTATG CTTTGTGTCT TGTAGGGGAT GCCATCTTGC TTTGCTGTGT TTGTCTATAC TGCTCAACAC CTAGGCAGCC CGTGAGATCT CGAAGCATCT CTCTTCTGCT TCTGGAGATC GTCAATGCGT GGTAACCTAC GCAGATCAGC TTAGTTAGTT ACCAATGTAG ACTACACAGG GTATGGAGGA GGATAGAATC CAGGTCCCTT TGGCATGCGA TGTCTGTGGC AGTTGTAATG GAGAACTCTG ACTTCTCGGC GGCCACATGG AGTGGTCTTT ACAAACGGTG ATGCAACTTC ACGATGGGAC GAGCAGTACT GGCCAGAGTG CAAGGCACCA GCACTCCTCT GGAGAGACGC GCAGCCAACG GGTTCGAATT AGACCTTGTG TTGGTGGAGA GTGGACTGAC CTTTGCAGCA ACAGGGGCTT TTGTATGACG CAAGCAGGCT TACTTAATGG GGATGGTCAT CGCTGTCCTG AGTAGTATCT GTATATACGT TTCCTGATCT AATTTTTTTC TCTTTACACT GTAATCCTTG AGGTCTTGGA AACTAGATTA TTTCAAACGT TTAATCATTG AAAAAGTTAC TT |
mRNA UTR + CDS |
| >Mp7g07270.1 AAGTGAGAAG CCGTGTTCAG TAGCGAGCAA GGTGAGAGCA GAGAGCGAGT CGTTAGCACG TCGATACATT GTCTTCTTGC AAGGCGGTCA TTCGTCTTCT TCGTCGTTCT TCAGGGCTTG CGGAGGGCAG TTTTCTTGAA GGCGTCCTCC AGCTCGAATT TAGTGTTCGT GTGCGTGGTG TTTGATCGGC GAAGGGAGAG ATTGACTGTG TGTTTGTGGG AGAGAGAGAG AGAGAGAGAG AGAGAGAGGA GCTCTTTGTG AGCCGGCCTG GCAGGCAAGG GCGAGGAAGG TAATCGAGGC CTTGTTCGCG GGCACCGGCG TGGCTTTAGT CGGTCTCGAG GGAGGAGCAG CAGCAGGAGC AGTTGGAGCG ATGGGGCGTT GTGGAATGCA GAGAACAAAT GTTTGGTTTT TGGTCCTTCT ATCGGTGTTA CTACTGATGG GGGTAGCGCA AGCCTCTTTT GACATGAGTT CTGAGACCTT CGTCCCACAG AGGACTGCTT CGTTGTCGAA TCAACTGGAC TCAGAGGTGA CTAGAAGAAT GCTGGCCGCC TCAGGGTACT ACGTCGGATA CGGTGCCTTA ACTGCCAATC GTGTTCCTTG CCCGCCTCAG TCGGGACGAT CGTATTACAC TCCCGGGTGC TCGACTGCTT CGGGACCAGT TCGGCCTTAC ACCAGAGGAT GCTCGACTAT CACCCGGTGT GCTAGGGATG GCTAGGTGCT TCGTTGAGAT TTCAAGGATG TCCGTTGACC TCCCTATTAT TTTCGGGGGA AGCCTGTTTG TTCCTTCCCT CCCACTGGAA AGAGGAACTA TTGTGAAGTG TTCGGGTTTC TCTCTCTTCT GCGCGGGGTG TTGGTGCTAA TACTGAAAGT CGGCAAACTC TCAAATAAAC CTTAAATAGT AAGAAAAAAA AGAAGTATAT TTCAGTTATG CTTTGTGTCT TGTAGGGGAT GCCATCTTGC TTTGCTGTGT TTGTCTATAC TGCTCAACAC CTAGGCAGCC CGTGAGATCT CGAAGCATCT CTCTTCTGCT TCTGGAGATC GTCAATGCGT GGTAACCTAC GCAGATCAGC TTAGTTAGTT ACCAATGTAG ACTACACAGG GTATGGAGGA GGATAGAATC CAGGTCCCTT TGGCATGCGA TGTCTGTGGC AGTTGTAATG GAGAACTCTG ACTTCTCGGC GGCCACATGG AGTGGTCTTT ACAAACGGTG ATGCAACTTC ACGATGGGAC GAGCAGTACT GGCCAGAGTG CAAGGCACCA GCACTCCTCT GGAGAGACGC GCAGCCAACG GGTTCGAATT AGACCTTGTG TTGGTGGAGA GTGGACTGAC CTTTGCAGCA ACAGGGGCTT TTGTATGACG CAAGCAGGCT TACTTAATGG GGATGGTCAT CGCTGTCCTG AGTAGTATCT GTATATACGT TTCCTGATCT AATTTTTTTC TCTTTACACT GTAATCCTTG AGGTCTTGGA AACTAGATTA TTTCAAACGT TTAATCATTG AAAAAGTTAC TT |
CDS |
| >Mp7g07270.1 ATGGGGCGTT GTGGAATGCA GAGAACAAAT GTTTGGTTTT TGGTCCTTCT ATCGGTGTTA CTACTGATGG GGGTAGCGCA AGCCTCTTTT GACATGAGTT CTGAGACCTT CGTCCCACAG AGGACTGCTT CGTTGTCGAA TCAACTGGAC TCAGAGGTGA CTAGAAGAAT GCTGGCCGCC TCAGGGTACT ACGTCGGATA CGGTGCCTTA ACTGCCAATC GTGTTCCTTG CCCGCCTCAG TCGGGACGAT CGTATTACAC TCCCGGGTGC TCGACTGCTT CGGGACCAGT TCGGCCTTAC ACCAGAGGAT GCTCGACTAT CACCCGGTGT GCTAGGGATG GCTAG |
Protein |
| >Mp7g07270.1 MGRCGMQRTN VWFLVLLSVL LLMGVAQASF DMSSETFVPQ RTASLSNQLD SEVTRRMLAA SGYYVGYGAL TANRVPCPPQ SGRSYYTPGC STASGPVRPY TRGCSTITRC ARDG |