Mecchia, M.A., et al. · 2022 · Development research
doi:10.1242/dev.200580 PMID:36178124
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp4g15890 | MpFER | experimental subject | The single CrRLK1L receptor kinase gene of M. polymorpha (also known as MpTHESEUS/MpTHE), characterized here as the ancestral member of the family. Knockdown (three independent amiR-MpFER lines, 10-20% of wild-type transcript) reduced thallus size, caused short bursting rhizoids and reduced male fertility (fewer and smaller antheridiophores), while CRISPR/Cas9 amorphic Mpfer knockout alleles showed far more severe loss of tissue integrity, reduced tissue stiffness and cell death, and MpFER overexpression disrupted epidermis, air-chamber and air-pore formation. proMpFER:trpVNS reporters show broad gametophyte expression including archegonia (not the egg cell), the zygote after fertilization, antheridia and sporophyte; AtFER could not complement Mpfer, indicating an ancestral, non-interchangeable role in cell expansion and cell-wall integrity maintenance. |
| Mp7g17560 | MpMRI | experimental subject | MARIS-orthologous receptor-like cytoplasmic kinase (also named MpPTI). A dominant-active proMpMRI:MpMRI-R240C construct transformed into the amiR-MpFER3-2 knockdown partially restored rhizoid growth, while high MpMRI expression itself produced epidermal/thallus abnormalities, placing MpMRI downstream of MpFER in a conserved CrRLK1L signalling cascade controlling polarized growth. |
| Mp5g09600 | MpLRE1 | sequence comparator | One of the two MpLRE/LLG (LORELEI-like) co-receptor genes of M. polymorpha; the protein conserves the eight Cys residues and the ND motif of the family but lacks a GPI-anchoring site, and was analysed for conservation of the RALF/CrRLK1L binding surface together with MpFER. |
| Mp4g22100 | MpLRE2 | sequence comparator | The second MpLRE/LLG co-receptor gene; conserves the eight family Cys residues, the ND motif and a GPI-anchoring site, and its predicted structure superimposes on AtLLG1, supporting a conserved MpFER co-receptor interface. Some MpLRE/MpRALF family members share the MpFER expression pattern. (This locus was later renamed MpLLG by Zhang et al. 2026, Nat. Plants.) |
| Mp7g07270 | MpRALF1 | sequence comparator | One of the three M. polymorpha RALF peptide genes analysed as candidate MpFER ligands; clusters with the AtRALF1 subgroup and carries the RRXL motif for S1P processing. NAMING NOTE: this paper's MpRALF numbering is swapped relative to the original naming. Bowman et al. 2017 (Cell 171:287-304) Table S60 registers MpRALF1 = Mapoly0076s0067 (Mp7g07270), MpRALF2 = Mapoly0040s0047 (Mp2g21670) and MpRALF3 = Mapoly0002s0166 (Mp1g27120); MarpolBase and Zhang et al. 2026 (Nat. Plants) follow that original naming. This locus (Mp7g07270) is therefore MpRALF1; the paper calls it MpRALF3. Linked by the ID stated in the paper, not by name. |
| Mp2g21670 | MpRALF2 | sequence comparator | One of the three M. polymorpha RALF peptides included in the phylogenetic and motif analysis of putative MpFER ligands; unlike the other two members it lacks the RRXL S1P-cleavage motif. |
| Mp1g27120 | MpRALF3 | sequence comparator | One of the three M. polymorpha RALF peptide genes analysed for conservation of the FER-binding surface; the mature peptides cluster with the AtRALF1 subgroup, share the four conserved Cys and the YXXY/YY motifs, and this member carries the RRXL motif required for S1P cleavage. NAMING NOTE: this paper's MpRALF numbering is swapped relative to the original naming. Bowman et al. 2017 (Cell 171:287-304) Table S60 registers MpRALF1 = Mapoly0076s0067 (Mp7g07270), MpRALF2 = Mapoly0040s0047 (Mp2g21670) and MpRALF3 = Mapoly0002s0166 (Mp1g27120); MarpolBase and Zhang et al. 2026 (Nat. Plants) follow that original naming. This locus (Mp1g27120) is therefore MpRALF3; the paper calls it MpRALF1. Linked by the ID stated in the paper, not by name. |
| Mp6g10990 | MpACT1 | experimental tool | One of three reference genes used to normalize ddPCR transcript measurements. |
| Mp6g11010 | MpACTIN7 | experimental tool | One of three reference genes used to normalize ddPCR transcript measurements. Gene ID not stated in the paper: assigned to Mp6g11010 (nomenclature symbol MpACTIN7), consistent with the MpACT7 ID stated in other papers of this corpus. |
| — | MpAPT3 missing | experimental tool | One of three reference genes used to normalize ddPCR transcript measurements. |
| Mp3g23400 | MpEF1 | experimental tool | Promoter used to drive the amiR-MpFER constructs and the proMpEF1:AtFER-Cit interspecific complementation construct, and used as a qRT-PCR reference gene. |
| Mp1g26670 | MpMIR160 | experimental tool | Endogenous microRNA precursor used as the backbone and template for the three artificial miRNA constructs targeting MpFER. |
| Mp1g17720 | Mp1g17720 missing | sequence background | Receptor-kinase locus reported as the closest M. polymorpha match (full-length and ectodomain) to the Closterium sexual-reproduction kinase CpRLK1, arguing that CpRLK1 is not a CrRLK1L ortholog. |
| Mp8g07990 | MpS1P | sequence background | Identified as the single M. polymorpha S1P (site-1 protease) ortholog, consistent with RRXL-motif processing of the MpRALF precursors. |