Mecchia, M.A., et al. · 2022 · Development research
doi:10.1242/dev.200580 PMID:36178124
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp4g15890 | MpFER | experimental subject | The single CrRLK1L receptor kinase gene of M. polymorpha (also known as MpTHESEUS/MpTHE), characterized here as the ancestral member of the family. Knockdown (three independent amiR-MpFER lines, 10-20% of wild-type transcript) reduced thallus size, caused short bursting rhizoids and reduced male fertility (fewer and smaller antheridiophores), while CRISPR/Cas9 amorphic Mpfer knockout alleles showed far more severe loss of tissue integrity, reduced tissue stiffness and cell death, and MpFER overexpression disrupted epidermis, air-chamber and air-pore formation. proMpFER:trpVNS reporters show broad gametophyte expression including archegonia (not the egg cell), the zygote after fertilization, antheridia and sporophyte; AtFER could not complement Mpfer, indicating an ancestral, non-interchangeable role in cell expansion and cell-wall integrity maintenance. |
| Mp7g17560 | MpMRI | experimental subject | MARIS-orthologous receptor-like cytoplasmic kinase (also named MpPTI). A dominant-active proMpMRI:MpMRI-R240C construct transformed into the amiR-MpFER3-2 knockdown partially restored rhizoid growth, while high MpMRI expression itself produced epidermal/thallus abnormalities, placing MpMRI downstream of MpFER in a conserved CrRLK1L signalling cascade controlling polarized growth. |
| Mp5g09600 | MpLRE1 | sequence comparator | One of the two MpLRE/LLG (LORELEI-like) co-receptor genes of M. polymorpha; the protein conserves the eight Cys residues and the ND motif of the family but lacks a GPI-anchoring site, and was analysed for conservation of the RALF/CrRLK1L binding surface together with MpFER. |
| Mp4g22100 | MpLRE2 | sequence comparator | The second MpLRE/LLG co-receptor gene; conserves the eight family Cys residues, the ND motif and a GPI-anchoring site, and its predicted structure superimposes on AtLLG1, supporting a conserved MpFER co-receptor interface. Some MpLRE/MpRALF family members share the MpFER expression pattern. (This locus was later renamed MpLLG by Zhang et al. 2026, Nat. Plants.) |
| Mp1g27120 | MpRALF1 paper
MpRALF3 nomenclature |
sequence comparator | One of the three M. polymorpha RALF peptide genes analysed for conservation of the FER-binding surface; the mature peptides cluster with the AtRALF1 subgroup, share the four conserved Cys and the YXXY/YY motifs, and this member carries the RRXL motif required for S1P cleavage. NAMING NOTE: this paper assigns MpRALF1 to Mp1g27120 (Mapoly0002s0166) and MpRALF3 to Mp7g07270 (Mapoly0076s0067), which is the OPPOSITE of the MarpolBase nomenclature (from the genome paper) and of Zhang et al. 2026 (Nat. Plants), where MpRALF1 = Mp7g07270 and MpRALF3 = Mp1g27120 (verified there by qPCR primer sequences). Linked here by the ID stated in this paper, not by name. |
| Mp2g21670 | MpRALF2 | sequence comparator | One of the three M. polymorpha RALF peptides included in the phylogenetic and motif analysis of putative MpFER ligands; unlike the other two members it lacks the RRXL S1P-cleavage motif. |
| Mp7g07270 | MpRALF3 paper
MpRALF1 nomenclature |
sequence comparator | One of the three M. polymorpha RALF peptide genes analysed as candidate MpFER ligands; clusters with the AtRALF1 subgroup and carries the RRXL motif for S1P processing. NAMING NOTE: the MpRALF1/MpRALF3 numbering used here is swapped relative to the MarpolBase nomenclature and to Zhang et al. 2026 (Nat. Plants), in which this locus (Mp7g07270) is MpRALF1. Linked by the ID stated in this paper. |
| Mp6g10990 | MpACT1 | experimental tool | One of three reference genes used to normalize ddPCR transcript measurements. |
| Mp6g11010 | MpACTIN7 | experimental tool | One of three reference genes used to normalize ddPCR transcript measurements. Gene ID not stated in the paper: assigned to Mp6g11010 (nomenclature symbol MpACTIN7), consistent with the MpACT7 ID stated in other papers of this corpus. |
| — | MpAPT3 missing | experimental tool | One of three reference genes used to normalize ddPCR transcript measurements. |
| Mp3g23400 | MpEF1 | experimental tool | Promoter used to drive the amiR-MpFER constructs and the proMpEF1:AtFER-Cit interspecific complementation construct, and used as a qRT-PCR reference gene. |
| Mp1g26670 | MpMIR160 | experimental tool | Endogenous microRNA precursor used as the backbone and template for the three artificial miRNA constructs targeting MpFER. |
| Mp1g17720 | missing | sequence background | Receptor-kinase locus reported as the closest M. polymorpha match (full-length and ectodomain) to the Closterium sexual-reproduction kinase CpRLK1, arguing that CpRLK1 is not a CrRLK1L ortholog. |
| Mp8g07990 | MpS1P | sequence background | Identified as the single M. polymorpha S1P (site-1 protease) ortholog, consistent with RRXL-motif processing of the MpRALF precursors. |