Mp4g22100
Description
Annotation
| Database | ID | Description |
|---|---|---|
| PANTHER | PTHR31533 | GPI-ANCHORED PROTEIN LLG1-RELATED-RELATED |
| MapolyID | Mapoly0090s0020 | - |
Nomenclature
| Gene symbol | Product | Transcript ID | Status |
|---|---|---|---|
| MpLRE2 | RALF co-receptor | Published | |
| MpLLG | LORELEI-like GPI-anchored protein | Published |
Associated Literature (AI-based literature mining, Beta-version)
GPI-anchored LORELEI-like co-receptor, renamed in this paper from MpLRE2 (the MpLRE gene that retains the GPI-anchoring site). MpLLG is enriched in antheridiophores and its Flag/eGFP fusions accumulate in sperm flagella; CRISPR Mpllg and amiRNA Ri-MpLLG lines showed reduced sperm ROS and motility that exogenous MpRALF1 failed to rescue. MpLLG co-immunoprecipitates with MpFERecd, binds MpRALF1 preferentially through the peptide C-terminal 38-aa region, and the MpLLG-N100A and MpLLG-A126Y substitutions disturb that binding, defining an MpRALF1-MpFER-MpLLG module upstream of ROS production. Gene ID not printed in the paper: resolved from the paper's own MpLLG-RT-F/R qPCR primers (Suppl. Table 2), which match Mp4g22100 (= MpLRE2 of Mecchia et al. 2022) exactly.
1 core 1 peripheral
Zhang, Y., et al. (2026)
· Nature Plants research experimental subject
GPI-anchored LORELEI-like co-receptor, renamed in this paper from MpLRE2 (the MpLRE gene that retains the GPI-anchoring site). MpLLG is enriched in antheridiophores and its Flag/eGFP fusions accumulate in sperm flagella; CRISPR Mpllg and amiRNA Ri-MpLLG lines showed reduced sperm ROS and motility that exogenous MpRALF1 failed to rescue. MpLLG co-immunoprecipitates with MpFERecd, binds MpRALF1 preferentially through the peptide C-terminal 38-aa region, and the MpLLG-N100A and MpLLG-A126Y substitutions disturb that binding, defining an MpRALF1-MpFER-MpLLG module upstream of ROS production. Gene ID not printed in the paper: resolved from the paper's own MpLLG-RT-F/R qPCR primers (Suppl. Table 2), which match Mp4g22100 (= MpLRE2 of Mecchia et al. 2022) exactly.
Mecchia, M.A., et al. (2022)
· Development research sequence comparator
The second MpLRE/LLG co-receptor gene; conserves the eight family Cys residues, the ND motif and a GPI-anchoring site, and its predicted structure superimposes on AtLLG1, supporting a conserved MpFER co-receptor interface. Some MpLRE/MpRALF family members share the MpFER expression pattern. (This locus was later renamed MpLLG by Zhang et al. 2026, Nat. Plants.)
Single-cell expression scRNA-seq atlas from Wang et al., 2023
Expression of Mp4g22100 across the single-cell RNA-seq atlas. Cluster identities (right) annotate the same UMAP used for the feature plot (left).
Gene structure:
Sequences: |
Gene UTR + CDS + intron |
| >Mp4g22100.1 ACGAATACAG GGCAAACGCT TTCTGGTGCC CCTTATAGCC CAGCGAGATT GGAGGATTCC CGTGGTCCAC ATTGCGAGAG TTTGAGGAGG CGGGAAGGTT TTTGTCGAGT GAATCCGTTC GAGGGTGGAG AGATTGCGTC CGTGAGAGAG GAAGAGAGGG GAGCGAGATC GGAAAGCTGC AGCGCGTGGG ATCGAGATCA GGGGACGGGT CTGAGAGAGA GTGACGGGAG TGGGGGAAGA GTGATTGTTC AATCGACTGG GACTGAACGT TTTTAACCGA CAGTGAGTGT CGAGCTGAGT CAACTGATAA GTGGTTGTTA GTGATTGTGA GGCATCCAGT TCATTCTTCA TATCGGGGAA GTGAGCCAGT GCATGATGGG AATGTCCAGA CCGTTGTATG CGATTGTGCT CGTGTGTTTG GTGTGCAGTA CGTTGGCCAA CTTTTCTAAT GGAGGTGAGG CCATTCTCGT CCTGACTTTC GGTTATGTGT TCTCTTCTGT AGAGCACTTT CCTTATCGAA GATTCGTATG GAATGTTTGA CTCCCGCTTC GATGCGATCA GTAAAATCCC GAGCATGTTT TTGGAGCTCA GTTCGTTTCC TTCTTCAGTC TGGCTCGACT TCATTTGAAA GACTTCTTCG TAGAAATGTG ACTGTTGTCT ACCAATGTAT GCCTCTGATA AGAAACAGTA CAGTACGGTT GGGGAGACGA GAGTAGTTCA GTCAGTCACA CGGATTGGGA TCAGTTTTGC AACTTGACTT CTTTCCGTGT TCTTTCGTGA AGAATGCAGT TTAGTATTCT GTTGGAACAA AGGTTTATTT CTGTGGTGCT ATCTAAATAC AACACAATGC ATTTGGGAAG CAACAACTAA AATTCTTGTT GATTGGACTC GCGGTTCCAC CGTGCTGTGT AATTCGGTTT TTCCTAACTA TGCAATGTGA CCGATACGAA TGCGTCTACC TGACGCGGGT AGAGCTTTGC GTGATCACTT GTTGGGATTT ATGAGCTGAA ATCTGACGTC AAACGGAATC GATTGCGGAG GTTTTGATGT AATTTCCTGG CTGTTACAGA GTTCGAAGCT AGTAAGGAAG ATTCCAGGAG TGGCGGGCGA CAGCTGTTGC AGAACAGTCA ATTACGTGAG TATACATTTG GCTTTTCTCT AGAGCGATGC ATTCTAGAAT ATGCGATCCT CATTGGGCCC TTTGGTGGGA TGAAGGTTTG TATGCTTGTA CGTTCTATTG GTCACTTTCC TCTGCAAGAA ATGATGCTGG ATTTAATCGG TTCCGCAGTA GTTGGAGGTC CTGTTATGTG AATGAGTCAA GAAATATGCC ACCACAGTCG GAAAGTTTTA TTTCTGGTAA TCTTCAGATG TTTACCATCA GCCGACTTTT ACACAGCATT CTCTAGACTT TGTCCAGCTC GTTACAAAGA CTCTTGTTTG GCTGTACGTG TTGTCTCTTC TTAGTATCTT CTCTGGCAGA ATTCAATGCC CCTAATGCCT CTGTGATGAT AAAGCTGTTG TGCTCTGTCT GCACTGATTG GAAATAATCT GGTCAAAATT TTTCTTTTTC TTGCTAAGTT CTCATAATAC CAATGTTCAA ATTTCTATAT AATACCCCGA AGTGCACTGG CATTTTCCGT AGTATATTCG TATTCCCACC CAAAGTTATC CTGATCATAC CTTTAAAACT CCAGTTGATC GTTGTTTACC ACGTCATATC GAAGCTCTGA CTTGAAGAGC TACCTATGCA CTTCAGAAAA TCTAGGGGAG AGGAAACTAA CTCGTGTCGT GTTTTTCTTT GTTGCAGCCT GTCCTGAAAA CTACAACTTC TTCAACTTCA CTCCAATTCT CACTGCGTGC CCCAGTGAGA ACAACATCTC CTTAGTCGGC ACGTTCAATG TCAAAAAGTG CTGTCAGTCT CTCTGCACAG TGCTTGGAGC AAATGGGAGC GTGATCAACG ATGCTAACTA TGATTGTGCC GAGCAGTTTT TCTCATACTT GAGCCTGGCA GGAAACTATA GTGGAGGATA TTTTGCAAGT ATATGTACTG ACCCTAATTC GCCTCAGGGT ATTTATTGTC CCCCAGTCGA GACAGCACCG CCACCAAGTA GCGCCTTTTC CGTAGGGGCC AGCCTTTGGT CGTTCCTTGT AGCGTTAGCA GTGTTCGAAG TACTAGTACT TGTAGTGTGA CATCCTGGTT TAAGTTGTCG GCATTTTGTT GCACACGTAG TCAGTAGATT CCGAAACCTG TAAAAATCAG GTCCCTGATT TTTGTTGTAA TAAGGCAAAC TTAATTAATT TTCCCTTTGA TATGCCAGG |
mRNA UTR + CDS |
| >Mp4g22100.1 ACGAATACAG GGCAAACGCT TTCTGGTGCC CCTTATAGCC CAGCGAGATT GGAGGATTCC CGTGGTCCAC ATTGCGAGAG TTTGAGGAGG CGGGAAGGTT TTTGTCGAGT GAATCCGTTC GAGGGTGGAG AGATTGCGTC CGTGAGAGAG GAAGAGAGGG GAGCGAGATC GGAAAGCTGC AGCGCGTGGG ATCGAGATCA GGGGACGGGT CTGAGAGAGA GTGACGGGAG TGGGGGAAGA GTGATTGTTC AATCGACTGG GACTGAACGT TTTTAACCGA CAGTGAGTGT CGAGCTGAGT CAACTGATAA GTGGTTGTTA GTGATTGTGA GGCATCCAGT TCATTCTTCA TATCGGGGAA GTGAGCCAGT GCATGATGGG AATGTCCAGA CCGTTGTATG CGATTGTGCT CGTGTGTTTG GTGTGCAGTA CGTTGGCCAA CTTTTCTAAT GGAGAGTTCG AAGCTAGTAA GGAAGATTCC AGGAGTGGCG GGCGACAGCT GTTGCAGAAC AGTCAATTAC CCTGTCCTGA AAACTACAAC TTCTTCAACT TCACTCCAAT TCTCACTGCG TGCCCCAGTG AGAACAACAT CTCCTTAGTC GGCACGTTCA ATGTCAAAAA GTGCTGTCAG TCTCTCTGCA CAGTGCTTGG AGCAAATGGG AGCGTGATCA ACGATGCTAA CTATGATTGT GCCGAGCAGT TTTTCTCATA CTTGAGCCTG GCAGGAAACT ATAGTGGAGG ATATTTTGCA AGTATATGTA CTGACCCTAA TTCGCCTCAG GGTATTTATT GTCCCCCAGT CGAGACAGCA CCGCCACCAA GTAGCGCCTT TTCCGTAGGG GCCAGCCTTT GGTCGTTCCT TGTAGCGTTA GCAGTGTTCG AAGTACTAGT ACTTGTAGTG TGACATCCTG GTTTAAGTTG TCGGCATTTT GTTGCACACG TAGTCAGTAG ATTCCGAAAC CTGTAAAAAT CAGGTCCCTG ATTTTTGTTG TAATAAGGCA AACTTAATTA ATTTTCCCTT TGATATGCCA GG |
CDS |
| >Mp4g22100.1 ATGATGGGAA TGTCCAGACC GTTGTATGCG ATTGTGCTCG TGTGTTTGGT GTGCAGTACG TTGGCCAACT TTTCTAATGG AGAGTTCGAA GCTAGTAAGG AAGATTCCAG GAGTGGCGGG CGACAGCTGT TGCAGAACAG TCAATTACCC TGTCCTGAAA ACTACAACTT CTTCAACTTC ACTCCAATTC TCACTGCGTG CCCCAGTGAG AACAACATCT CCTTAGTCGG CACGTTCAAT GTCAAAAAGT GCTGTCAGTC TCTCTGCACA GTGCTTGGAG CAAATGGGAG CGTGATCAAC GATGCTAACT ATGATTGTGC CGAGCAGTTT TTCTCATACT TGAGCCTGGC AGGAAACTAT AGTGGAGGAT ATTTTGCAAG TATATGTACT GACCCTAATT CGCCTCAGGG TATTTATTGT CCCCCAGTCG AGACAGCACC GCCACCAAGT AGCGCCTTTT CCGTAGGGGC CAGCCTTTGG TCGTTCCTTG TAGCGTTAGC AGTGTTCGAA GTACTAGTAC TTGTAGTGTG A |
Protein |
| >Mp4g22100.1 MMGMSRPLYA IVLVCLVCST LANFSNGEFE ASKEDSRSGG RQLLQNSQLP CPENYNFFNF TPILTACPSE NNISLVGTFN VKKCCQSLCT VLGANGSVIN DANYDCAEQF FSYLSLAGNY SGGYFASICT DPNSPQGIYC PPVETAPPPS SAFSVGASLW SFLVALAVFE VLVLVV |