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Functions of COP1/SPA E3 Ubiquitin Ligase Mediated by MpCRY in the Liverwort Marchantia Polymorpha under Blue Light.

Zhang, L., et al. · 2022b · International Journal of Molecular Sciences   research

doi:10.3390/ijms23010158   PMID:35008588   PMC8745113

Linked genes (8)  4 core 4 peripheral

Gene ID Name Evidence / Role Function (this paper)
Mp1g16800 MpBZIP1 experimental subject Ortholog of AtHY5 (Mp1g16800) and target of the COP1/SPA complex; its C-terminus is bound by the MpSPA coiled-coil domain. MpHY5 is ubiquitinated and destabilized via MpCOP1/MpSPA, but does not regulate symmetric thallus growth.
Mp5g12010 MpCOP1 experimental subject E3 ubiquitin ligase with RING-finger, coiled-coil, and WD40 domains; interacts with full-length MpSPA. It ubiquitinates MpHY5, a reaction promoted by MpSPA; CRISPR knockout lines were lethal, limiting full characterization.
Mp2g17590 MpCRY experimental subject Central blue-light receptor of the study; knockout lines Mpcry-7 and Mpcry-8 were generated by CRISPR/Cas9. MpCRY is degraded and phosphorylated under blue light like Arabidopsis CRY2, and constitutively interacts with MpSPA in yeast.
Mp3g25460 MpSPA1 experimental subject COP1-interacting regulator with kinase-like, coiled-coil, and WD40 domains; knockout lines Mpspa-8 and Mpspa-11 were generated. MpSPA accumulates under blue light and promotes MpCOP1-mediated ubiquitination of MpHY5.
Mp3g14360 MpFKF citation background ZEITLUPE-family blue-light receptor cited as background in the M. polymorpha photoreceptor repertoire.
Mp5g03810 MpPHOT citation background Phototropin blue-light receptor cited among previously known M. polymorpha photoreceptors.
Mp2g16090 MpPHY citation background Single-copy phytochrome ortholog cited as previously characterized photoreceptor context in M. polymorpha.
Mp2g11590 MpUVR8 citation background UV-B photoreceptor cited as part of the M. polymorpha light-signaling background.