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An enhancer trap system to track developmental dynamics in Marchantia polymorpha.

Marron, A. O., et al. · 2023 · The Plant Journal   research

doi:10.1111/tpj.16394   PMID:37583263   PMC10952768

Linked genes (11)  0 core 11 peripheral

Gene ID Name Evidence / Role Function (this paper)
Mp4g21300 Mp1R-MYB17 sequence subject Candidate gene near the insertion site of the margin tissue marker line ET239-P64. Noted from RNAseq to be strongly up-regulated by auxin (2,4-D) treatment.
Mp4g00190 Mp4g00190 missing sequence subject Candidate gene near the ET239-P54 insertion site, encoding a small protein of unknown function with no known homology to other plant genes.
Mp4g04240 Mp4g04240 missing sequence subject Candidate gene near the insertion site of the apical notch marker line ET239-P161. BLAST shows similarity to an embryophyte-specific gene family found across all land plants.
Mp4g21310 Mp4g21310 missing sequence subject Candidate gene near the ET239-P64 margin marker insertion site, encoding a protein of unknown function, identified by genomic analysis.
Mp4g21320 Mp4g21320 missing sequence subject Candidate gene near the ET239-P64 margin marker insertion site, annotated as a cyclophilin-type peptidyl-prolyl isomerase, identified by genomic analysis.
Mp3g17930 MpRSL1 sequence subject Identified by genomic analysis as the candidate gene near the insertion site of the rhizoid marker line ET239-P177. Annotated as the Marchantia homolog of Root Hairless-like 6 (RSL6), a transcription factor implicated in rhizoid/root-hair development.
Mp4g00200 MpWRKY12 sequence subject Candidate gene near the insertion site of the oil cell marker line ET239-P54, found enriched in the oil cell cluster. A WRKY-family transcription factor consistent with oil cells' role in anti-herbivore defense.
Mp4g00180 MpWRKY13 sequence subject WRKY-family candidate gene located near the ET239-P54 oil cell marker insertion site, identified via genomic analysis of the trapped region.
Mp3g21660 MpPIN1 experimental tool Used as a marker line to visualize polar auxin transport; elevated auxin treatment increased the spatial distribution of MpPIN1 expression as predicted.
Mp8g08780 MpYUC2 experimental tool Used as a validation marker line; auxin and inhibitor treatments confirmed the expected response, demonstrating treatments did not non-specifically alter enhancer trap signals.
Mp5g06970 MpERF20 citation background Cited from prior work: over-expression of MpLAXR (MpERF20) caused strong cell division at gemma edges, discussed as context for margin tissue regulation.