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Nanoclustering of a plant transcription factor enables strong yet specific DNA binding.

Arfman K, Janssen BPJ, Romein R, van den Boom S, van der Woude M, Jansen L, Rademaker M, Hernández-García J, Ramalho JJ, Dipp-Álvarez M, de Roij M, Lamers J, Hohlbein J, Borst JW, Weijers D, van Mierlo CPM, Sprakel J. · 2026 · Sci Adv   research

doi:10.1126/sciadv.aed7904   PMID:42497281   PMC13398500

Linked genes (2)  1 core 1 peripheral

Gene ID Name Evidence / Role Function (this paper)
Mp4g11820 MpARF2 experimental subject Auxin response factor shown to form nanoscopic clusters in the nucleus rather than acting as a mono/oligomer or forming phase-separated condensates. Nanoclustering gives MpARF2 high-affinity, switch-like and sequence-specific DNA binding that neither of the other two modes provides.
Mp3g23400 MpEF1 experimental tool Used together with MpSAND as a reference gene for RT-qPCR normalisation.