MarpolBase - Comparison of the MpEF1alpha and CaMV35 promoters for application in Marchantia polymorpha overexpression studies.

Comparison of the MpEF1alpha and CaMV35 promoters for application in Marchantia polymorpha overexpression studies.

Althoff F, Kopischke S, Zobell O, Ide K, Ishizaki K, Kohchi T, Zachgo S. · 2014 · Transgenic Res research

Gene ID	Name	Evidence / Role	Function (this paper)
Mp3g23400	MpEF1	experimental subject	Endogenous elongation factor 1-alpha promoter characterized as a constitutive driver for Marchantia overexpression/silencing; the proMpEF1α::GUS reporter showed strong meristematic and broadly ubiquitous expression, outperforming CaMV35S. Whole-mount in situ hybridization of endogenous MpEF1α mRNA matched the GUS pattern (meristematic zones of gemmae; egg cells, neck and venter cells of young archegonia).
Mp4g13430	MpH4 paper MpH4c nomenclature	experimental tool	Marchantia Histone H4 homologue (MpHistone4 / MpH4), isolated here as a cell-division marker; used as an antisense in-situ probe to validate the whole-mount in situ hybridization method. Its signal marked dividing cells around the meristematic zones and gemma margins. Used as a tool/marker, not the subject of study.