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Identification of Reference Genes for Real- Time Quantitative PCR Experiments in the Liverwort Marchantia polymorpha.

Saint-Marcoux, D., et al. · 2015 · PLoS ONE   research

doi:10.1371/journal.pone.0118678   PMID:25798897   PMC4370483

Linked genes (13)  0 core 13 peripheral

Gene ID	Name	Evidence / Role	Function (this paper)
—	MpACT missing	experimental tool	Candidate reference gene with one of the lowest z-scores; recommended together with MpAPT as a stable normalizer.
Mp3g25140	MpAPT	experimental tool	Candidate reference gene with one of the lowest z-scores; most suitable and recommended for generic qPCR normalization.
—	MpCUL missing	experimental tool	Candidate reference gene showing moderate expression stability across the conditions tested.
Mp3g23400	MpEF1	experimental tool	Candidate reference gene; expression-stable and suitable for normalization in developmental comparisons.
—	MpELF5 missing	experimental tool	Candidate reference gene showing moderate expression stability across the conditions tested.
—	MpGAPC1 missing	experimental tool	Candidate reference gene with high transcript variability across groups; unsuitable as a reference gene.
—	MpH3 missing	experimental tool	Candidate reference gene with low variation under hormone treatment but high variation in other contexts.
—	MpNRT2 missing	experimental tool	Nitrate transporter used as a target to demonstrate normalization accuracy; induced ~14-27 fold under nitrate starvation.
—	MpPEX missing	experimental tool	Candidate reference gene showing moderate expression stability across the conditions tested.
—	MpPHT1 missing	experimental tool	Phosphate transporter used as a target to validate normalization; induced ~4-5 fold under phosphate starvation.
Mp4g07110	MpSAND missing	experimental tool	Candidate reference gene suitable for development and abiotic stress but elevated after hormone treatments.
—	MpTUB8 missing	experimental tool	Candidate reference gene showing large expression variation; unsuitable for qPCR normalization.
—	MpUBQ10 missing	experimental tool	Candidate reference gene whose transcript levels varied most; judged unsuitable for qPCR normalization.