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Ancestral function of the phytochelatin synthase C-terminal domain in inhibition of heavy metal-mediated enzyme overactivation Journal of Experimental Botany

Li, M., et al. · 2020e   research

doi:10.1093/jxb/eraa386   PMID:32936292   PMC7586750

Linked genes (3)  1 core 2 peripheral

Gene ID	Name	Evidence / Role	Function (this paper)
Mp7g00960	MpPCS	experimental subject	Phytochelatin synthase isolated and cloned from M. polymorpha; recombinant enzyme catalyzed PCn synthesis from GSH, expression overexpression fully complemented the PC-deficient Arabidopsis cad1-3 mutant, and yeast expressing it tolerated 100 uM CdSO4. Acts as the central functionally studied gene, expressed constitutively with stronger thallus-midrib expression.
Mp6g11010	MpACTIN7	experimental tool	Used as a reference/internal control gene for RT-PCR normalization in M. polymorpha.
Mp3g25140	MpAPT	experimental tool	Used as a reference gene for expression normalization in M. polymorpha (stability 1.19).