Kanazawa, T., et al. · 2022 · Frontiers in Plant Science research
doi:10.3389/fpls.2022.979066 PMID:36046592 PMC9420845
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| — | MpRET1 missing | experimental subject | Alpha COPI subunit; tested by beta-estradiol-inducible knockdown with significantly reduced mRNA. Knockdown caused abnormally shaped oil bodies and severe developmental inhibition, consistent with COPI dysfunction in oil body formation. |
| — | MpSEC21 missing | experimental subject | Gamma COPI subunit; tested by beta-estradiol-inducible knockdown with qRT-PCR-confirmed reduced mRNA. Knockdown produced abnormally shaped oil bodies and severely impaired growth (more severe than loss of MpSEC28), supporting a COPI role in oil body morphogenesis. |
| Mp1g28220 | MpSEC28 missing | experimental subject | Causal gene of the screened T-DNA mutant; encodes the epsilon COPI subunit (T-DNA in 5' UTR of Mp1g28220). Mpsec28 mutants show rounded oil bodies (circularity 0.82 vs WT 0.45). MpSEC28-mCherry forms ring-shaped structures at the Golgi periphery, colocalizing with COPI markers. |
| Mp3g25140 | MpAPT | experimental tool | Used as the internal reference gene for qRT-PCR normalization of target transcript levels. |
| — | MpERD2 missing | experimental tool | Alternative Golgi marker (MpERD2-mCitrine); ring-like MpSEC28 structures associated with the Golgi periphery labeled by it. |
| Mp1g19070 | MpGOS11 | experimental tool | Golgi apparatus marker (mCitrine-MpGOS11); used for colocalization showing MpSEC28-mCherry localizes to COPI-coated vesicles at the Golgi periphery. |
| — | MpIDH1 missing | experimental tool | Mitochondrial marker (MpIDH1-mCitrine) used as negative control; low colocalization with MpSEC28 confirmed absence from mitochondria. |
| Mp1g08940 | MpRAB5 | experimental tool | Multivesicular body marker (mCitrine-MpRAB5); no clear colocalization with MpSEC28, excluding MVB localization. |
| Mp4g20670 | MpSYP12B | experimental tool | Qa-SNARE targeted to the oil-body membrane; mGFP-MpSYP12B lines used as the oil-body membrane marker to observe oil body morphology in mutants and knockdowns. |
| Mp2g14140 | MpSYP4 | experimental tool | Trans-Golgi network marker (mCitrine-MpSYP4); low Pearson colocalization with MpSEC28-mCherry indicated MpSEC28 is not at the TGN. |
| Mp3g02320 | MpC1HDZ | citation background | Transcription factor that, together with MpERF13, mediates oil body cell differentiation and oil body development in M. polymorpha; upstream regulator of the process studied. |
| Mp6g08690 | MpERF13 | citation background | Transcription factor mediating oil body cell differentiation and oil body development in M. polymorpha; cited as upstream regulatory machinery. |
| Mp2g19600 | MpSYP13B | citation background | Plasma-membrane-resident Qa-SNARE homologous to MpSYP12B that mediates secretion to the extracellular space, contrasting with MpSYP12B redirection to the oil body. |